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Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
(American Chemical Society, 2014-05)
The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant ...
Single-chain antibody fragments: Purification methodologies
(Elsevier, 2013-06)
At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical ...
F(ab')2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement
(Sociedad de Biología de Chile, 2005)
Cultivation of Pichia pastoris carrying the scFv anti LDL (−) antibody fragment. Effect of preculture carbon source
(Sociedade Brasileira de Microbiologia, 2017-07)
Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions ...
Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
(Associação Brasileira de Divulgação Científica, 2000)
We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple ...
Production of endoglin-specific heavy chain antibody fragments (VHHs) microarrays for whole-cell SPR imaging
(Elsevier, 2022)
Cell-based Surface Plasmon Resonance imaging (SPRi) with anti-endoglin VHH antibodies microarray on a gold-covered biochip was used to characterize the binding to endoglin expressed on the surface membrane of SC cells. ...
F(ab')2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement
(Society of Biology of Chile, 2005)
Trypanosoma cruzi calreticulin (TcCRT), described in our laboratory, retains several important functional features from its vertebrate homologues. We have shown that recombinant TcCRT inhibits the human complement system ...